Amine Miled, B.E, M.A.Sc, Ph.D.
Université Laval, Québec, Canada
LABioTRON, Bioengineering Research Laboratory
www.labiotron.com

Research Expertise
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  1. Brain Chemical Disorder "Study of the effect of neurotransmitter concentrations on the local conductivity and impedance of the cerebrospinal fluid in addition to their biological effects. Different neurotransmitters are studied such as Glutamate and Gamma-Aminobutyric acid (GABA), which are the major excitatory and inhibitory neurotransmitters respectively, and dopamine, which is involved in many psychiatric disorders such as Parkinson’s disease, drug’s addiction and schizophrenia. We used different concentrations of artificial cerebrospinal fluid (ACSF) since its conductivity is too high. Obtained results prove that neurotransmitter concentration can be detected by sensing the conductivity and the impedance of the ACSF", Extracted and adapted fro6th International IEEE EMBS Conference on Neural Engineering

  2. Observed Dopamine Neurotransmitter with in vitro experiment (Blue dot)

  3. Microfluidic "Platform for bioparticles mixing and detection in Lab-on-chip (LoC) based device dedicated to neurotransmitters analysis. The proposed biosensing device is characterized by a low voltage dielectrophoretic separation using novel L-shape electrodes. In addition to the mixing architecture enabling reaction between different particles, liquid was also sampled using the same electrodes. Our design works with different low voltages depending on particle size. It is tested with microspheres in the range of micrometers with an applied voltage less than 5V. The system dimensions ar e reduced to the minimum size such as it needs only few picolitre liquid samples. In addition to have a better control and separation, it is crucial to design many in-channel electrodes with minimum dimensions. Thus the microchannel includes 32 L-shape electrodes. The width of each electrode is 10 μm separated by 10 μm. Consequently, the width of the microchannel is 650 μm. The number of electrodes was choosen based on the number of outputs available on the monitoring electrical circuit." NEWCAS'10


  4.       
    Various realized microfluidic design


  5. New Packaging Techniques for Hybrid Microsystems New packaging technique in a hybrid microelectronics / microfluidic platform dedicated for Lab-on-Chip (LoC) based devices, which are intended for implantable medical microsystems. One of main features of the proposed LoC is its thickness that must be very small, including microchannels and complementary-metal-oxide semiconductor (CMOS) chip. This chip is integrated into the glass top-layer of the LoC whose thickness is 0.5 mm. The size of the device is 5 mm x  5 mm. Two microfluidic architectures were tested, one of them includes 81 square electrodes with a 30 um channel width, and the second is composed of 1622 L-shaped electrodes in a 650 um channel width. The packaging was tested using the square electrode arrays, owing to fabrication restrictions. The 64 L-shaped electrodes were connected to a printed circuit board for cell manipulation using dielectrophoresis in the objective to be connected to a CMOS chip in the bottom side of the microfluidic chip, owing to the large space needed for routing. The proposed platform also includes a new polydimethylsiloxane (PDMS)-based packaging technique to protect the integrated circuit (IC) from liquid leakage, in addition to a fast tubing process. The designed LoC requires only 2 picoliters as sample volume. Furthermore, by using the same layer where microchannels and IC are integrated, we succeeded the packaging of the whole LoC. Different packaging scenarios were tested using ultraviolet light and conventional epoxy, as well as PDMS and commercial connectors. The proposed packaging technique demonstrated the smallest size and the most efficient assembly method.

    Example of realized LoC packaging

  6. New Low-Cost and Fast Micro-fabrication Methods for Lab-on-chip Devices (Micro-electrode Array, Micro-channels...) "Microfluidic system such as a lab-on-chip (LOC) and a method of producing thereof. The system has an interconnector for connecting a microfluidic structure to a controller. There is a microfluidic structure having at least one electrode that can be connected with an interconnector to an electronic circuit. The interconnector has at least one electrical contact corresponding to the pin-outs of the electronic circuit for replaceably connecting them thereto. Each of the at least one electrical contact is connected to a corresponding one of the at least one electrode. The microfluidic structure and the electronic circuit in a combined configuration form together a re-usable and/or reconfigurable microfluidic system." Pending Patent  WO/2012/155266  

    Designed electrodes

  7. Lab-on-Chip "hybrid microelectronics / microfluidic Lab-on-Chip (LoC) platform intended for implantable medical microsystems for neurotransmitter detection. In vitro experiments were achieved using artificial cerebrospinal fluid (ACSF) from Tocris Bioscience where microspheres were immersed to test the behaviour of the designed LoC. One of main features of the proposed LoC platform is its thin thickness, including micro-channels and silicon CMOS chip. The latter is integrated into the glass top-layer of the LoC measuring 0.5 mm. The size of the device is 9 mm × 5 mm. the electrode architecture is composed of 8×2×2 L-shaped electrodes in a 650 μm channel width and 4 sites for interdigitited electrodes. 32 L-shaped electrodes were connected to a electronics circuit for cells manipulation using dielectrophoresis (DEP). The described LoC achieved an efficient separation within a concentration of 50 μl of a solution of microspheres, distilled water (DW) and 500 μl of ACSF. Beyond this concentration, electrode destruction was observed." EMBC'12

  8. Microsystem Design New CMOS interface for cell manipulation by dielectrophoresis and capacitive sensing system dedicated for a lab-on-a-chip. It fully integrates a signal generation circuit and a post-processing system to control parameters of each signal such as frequency, phase and amplitude. In addition, a large capacitive and low resistive load driver circuit is designed to deliver a current of 9 mA for each 16 electrodes as the microfluidic architecture is divided into 4 blocs containing 16 electrodes each one. Thus, the proposed CMOS chip provides 4-channel signals with individually controllable phase and amplitude. In addition, a capacitive sensing system has been integrated into the same chip to detect the capacitive change in the microchannel in the Lab-on-a-chip. The generated signals have a 2.5 peak-to-peak voltage range and 70 kHz frequency range while the detection system has a dynamic range of 1.5 V and a sensitivity of 11.8 fF/V." ISCAS'11


    Designed 1st fully assembled LoC

  9. Dielectrophoretic manipulations "a low voltage fully integrated Laboratory-on-Chip (LoC) for dielectrophoretic manipulation and capacitive sensing of nano and micro particles is introduced. The proposed system is intended to design an implantable LoC. The lowest static power consumption of the implemented Integrated circuit is 650 μA with a voltage supply of −1.10 and +1.8 V. Three different sizes of carboxyl-modified polystyrene particles (diameters of 0.22, 0.97 and 2.04 μm) where tested experimentally with three different electrode architectures to achieve dielectrophoretic mixing and separation. U-shaped, L-shaped and octagonal electrodes are used to perform the separation and mixing operations. The biosensing part is designed with a charge based capacitive sensor with an integrated sigma-delta modulator at its output stage. It was tested experimentally with algae and ethanol. The chip size is 1.2 by 1.2 mm and it is connected to a 15 × 30 cm microfluidic design. An efficient particle manipulation was achieved by applying a voltage of 1.7 V peak to peak in the microchannel with 90 and 180° dephased signals."

  10. Mixed and Analog Circuit Design "fully integrated CMOS interface dedicated for cells manipulation and separation in LoC devices. The proposed interface includes all microelectronics circuits for dielectrophoretic manipulation and capacitive detection. It contains also an embedded frequency and phase micro controller to ensure several kinds of cells displacements such as rotation, translation and separation with a wide tunable frequency band of 250 kHz for LoC applications. Furthermore, the detection part is a charge-based capacitive measurement circuit with a high sensitivity of 10 mV for each 1 fF. This new chip is a fully integrated CMOS interface into a LoC device including both the microfluidic and the microelectronics structures. It represents one of the first microfluidic processors." ASQED'10

  11. Integrated Sensors and Actuators "New Lab-on-Chip (LoC) architecture for dielectrophoresis-based cell manipulation, detection, and capacitive measurement. The proposed LoC is built around a CMOS full-custom chip and a microfluidic structure. This CMOS chip is used to deliver all parameters required to control the dielectrophoresis (DEP) features such as frequency, phase, and amplitude of signals spread on in-channel electrodes of the LoC. Based on a variable dynamic range sensing array, this chip integrates also an elaborated processing module enhancing the analysis time. The present work, introduce the proposed CMOS chip that is integrated to the LoC and presents experimental results related to micro and nano particles manipulation and detection in a microfluidic platform using the proposed integrated circuit. Thus, the proposed microsystem includes an on-chip 27-bit frequency divider, a digital phase controller with a 3.6 phase shift resolution and a 2.5 V dynamic range. The sensing module is composed of a 3  3 capacitive sensor array with 10 fF per mV sensitivity, and a dynamic range of 1.5 V . The measurements are digitally retrieved from the sensors using a Sigma-Delta analog-to-digital converter. The proposed LoC is intended for a self-driven device with different particles DEP manipulation including mixing, separation and trapping depending on the application in addition to an advanced sensing architecture. Thus, a fully automated CMOS chip is designed to be controlled by an FPGA. The obtained results show an efficient nano and micro-particles (PC05N, PA04N and PS03N) separation based on frequency segregation with low voltages less than 1.7 V and a fully integrated and reconfigurable system. The designed system is intended for neurotransmitters detection and separation after being validated by nano and micro-particles.", IEEE TBiocas, Pages: 120-132, Vo. 6, No. 2, 2012

    Particle manipulation in artificial cerebrospinal fluid

  12. Particle displacement modelling in microchannel "New modeling approach for Dielectrophoresis (DEP) based particle manipulation is presented. The proposed method fulfills missing links in finite element modeling between the multiphysic simulation and the biological behavior. This technique is amongst the first steps to develop a more complex platform covering several types of manipulations such as magnetophoresis and optics. The modeling approach is based on a hybrid interface using both ANSYS and MATLAB to link the propagation of the electrical field in the micro-channel to the particle motion. ANSYS is used to simulate the electrical propagation while MATLAB interprets the results to calculate cell displacement and send the new information to ANSYS for another turn. The beta version of the proposed technique takes into account particle shape, weight and its electrical properties. First obtained results are coherent with experimental results." MDPI Sensors'13